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Article

Generation of human TRIM5alpha mutants with high HIV-1 restriction activity.

Gene therapy - 1 Jul 2010

Pham Q T, Bouchard A, Grütter M G, Berthoux L

Abstract excerpt

Rhesus macaque tripartite motif (TRIM)5alpha potently inhibits early stages of human immunodeficiency virus (HIV)-1 replication, while the human orthologue has little effect on this virus. We used PCR-based random mutagenesis to construct a large library of human TRIM5alpha variants containing mutations in the PRYSPRY domain. We then applied a functional screen to isolate human cells made resistant to HIV-1...

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Identifiers

PMID: 20357830
DOI: 10.1038/gt.2010.40

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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