Article

Decoy-seq unlocks scalable genetic screening for regulatory small noncoding RNAs

2025-01-27

Abstract excerpt

Small noncoding RNAs (smRNAs) play critical roles in regulating various cellular processes, including development, stress response, and disease pathogenesis. However, functional characterization of smRNAs remains limited by the scale and simplicity of phenotypic readouts. Recently, single-cell perturbation screening methods, which link CRISPR-mediated genetic perturbations to rich transcriptomic profiling, have em...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d6021c16-068c-5733-86e7-3ab74f975c45
DOI: 10.1101/2025.01.25.634869

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Decoy-seq unlocks scalable genetic screening for regulatory small noncoding RNAsDOI 10.1101/2025.01.25.634869Read

Massively Targeted Evaluation of Therapeutic CRISPR Off-Targets in CellsSemantic proximity Read

Functional landscape of circular RNAs in human cancer cellsSemantic proximity Read

High-resolution characterization of gene function using single-cell CRISPR tiling screen.Semantic proximity Read

EPIKOL, a chromatin-focused CRISPR/Cas9-based screening platform, to identify cancer-specific epigenetic vulnerabilitiesSemantic proximity Read

Long noncoding RNA repertoire and targeting by nuclear exosome, cytoplasmic exonuclease, and RNAi in fission yeast.Semantic proximity Read

Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targetingSemantic proximity Read

The Dark Genome Investigating Pseudogenes and Non-Coding Regions in Genetic RegulationSemantic proximity Read

Genome-wide annotation of gene regulatory elements linked to cell fitnessSemantic proximity Read

Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences.Semantic proximity Read

Live-Cell Covalent Profiling Reveals Principles of RNA-Small Molecule Recognition across the Human TranscriptomeSemantic proximity Read

High Precision Quantification of small RNA Slicing Activity - Native Index Ligation-based Targeted Degradome Sequencing (NIL-TDS)Semantic proximity Read

Machine learning methods for predicting guide RNA effects in CRISPR epigenome editing experimentsSemantic proximity Read