Article

High Precision Quantification of small RNA Slicing Activity - Native Index Ligation-based Targeted Degradome Sequencing (NIL-TDS)

2025-10-04

Abstract excerpt

<h4>ABSTRACT</h4> RNA interference (RNAi) is an effective and precise regulatory mechanism in eukaryotes in which small RNAs mediate endonucleolytic slicing of complementary target mRNAs. Despite the potential of RNAi for human therapeutics and crop bio-protection, analytical platforms to quantitatively validate the slicing activities of small RNAs remain limited. Here, we present NIL-TDS, a cost-effective method...

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Literature Corpus work: 3c05866d-65f9-5507-a361-0af9258aa82b
DOI: 10.1101/2025.09.30.679503

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