Article
High-resolution characterization of gene function using single-cell CRISPR tiling screen.
Nature communications - 1 Jul 2021
Yang Lu, Chan Anthony K N, Miyashita Kazuya, Delaney Christopher D, Wang Xi, Li Hongzhi, Pokharel Sheela Pangeni, Li Sandra, Li Mingli, Xu Xiaobao, Lu Wei, Liu Qiao, Mattson Nicole, Chen Kevin Yining, Wang Jinhui, Yuan Yate-Ching, Horne David, Rosen Steven T, Soto-Feliciano Yadira, Feng Zhaohui, Hoshii Takayuki, Xiao Gang, Müschen Markus, Chen Jianjun, Armstrong Scott A, Chen Chun-Wei
Abstract excerpt
Identification of novel functional domains and characterization of detailed regulatory mechanisms in cancer-driving genes is critical for advanced cancer therapy. To date, CRISPR gene editing has primarily been applied to defining the role of individual genes. Recently, high-density mutagenesis via CRISPR tiling of gene-coding exons has been demonstrated to identify functional regions in genes. Furthermore,...
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