Article

A nanobody-based strategy for rapid and scalable purification of native human protein complexes

2023-03-10

Abstract excerpt

Native isolation of proteins in high yield and purity is a major bottleneck for analysis of their three- dimensional structure, function, and interactome. Here, we present a streamlined workflow for the rapid production of proteins or protein complexes using lentiviral transduction of human suspension cells, combined with highly-specific nanobody-mediated purification and proteolytic elution. (1) First, generation...

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Literature Corpus work: 4feb4da9-4255-5588-a6cb-99cd06a27381
DOI: 10.1101/2023.03.09.531980

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