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Detection of genome-wide low-frequency mutations with Paired-End and Complementary Consensus Sequencing (PECC-Seq) revealed end-repair derived artifacts as residual errors

2019-12-23

Abstract excerpt

<h4>ABSTRACT</h4> To improve the accuracy and the cost-efficiency of next-generation sequencing in ultralow-frequency mutation detection, we developed the Paired-End and Complementary Consensus Sequencing (PECC-Seq), a PCR-free duplex consensus sequencing approach. PECC-Seq employed shear points as endogenous barcodes to identify consensus sequences from the overlap in the shortened, complementary DNA strands-der...

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Literature Corpus work: d405fd03-ce7b-5c69-82a5-8ac6f611468d
DOI: 10.1101/2019.12.22.886440

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