Article
Detection of genome-wide low-frequency mutations with Paired-End and Complementary Consensus Sequencing (PECC-Seq) revealed end-repair-derived artifacts as residual errors.
Archives of toxicology - 1 Oct 2020
You Xinyue, Thiruppathi Suresh, Liu Weiying, Cao Yiyi, Naito Mikihiko, Furihata Chie, Honma Masamitsu, Luan Yang, Suzuki Takayoshi
Abstract excerpt
To improve the accuracy and the cost-efficiency of next-generation sequencing in ultralow-frequency mutation detection, we developed the Paired-End and Complementary Consensus Sequencing (PECC-Seq), a PCR-free duplex consensus sequencing approach. PECC-Seq employed shear points as endogenous barcodes to identify consensus sequences from the overlap in the shortened, complementary DNA strand-derived paired-end...
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