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Article

Ultrasensitive and high-efficiency screen of de novo low-frequency mutations by o2n-seq.

Nature communications - 22 May 2017

Wang Kaile, Lai Shujuan, Yang Xiaoxu, Zhu Tianqi, Lu Xuemei, Wu Chung-I, Ruan Jue

Abstract excerpt

Detection of de novo, low-frequency mutations is essential for characterizing cancer genomes and heterogeneous cell populations. However, the screening capacity of current ultrasensitive NGS methods is inadequate owing to either low-efficiency read utilization or severe amplification bias. Here, we present o2n-seq, an ultrasensitive and high-efficiency NGS library preparation method for discovering de novo,...

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Identifiers

PMID: 28530222
DOI: 10.1038/ncomms15335
PMCID: PMC5458117

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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