Article
Single duplex DNA sequencing with CODEC detects mutations with high sensitivity.
Nature genetics - 1 May 2023
Bae Jin H, Liu Ruolin, Roberts Eugenia, Nguyen Erica, Tabrizi Shervin, Rhoades Justin, Blewett Timothy, Xiong Kan, Gydush Gregory, Shea Douglas, An Zhenyi, Patel Sahil, Cheng Ju, Sridhar Sainetra, Liu Mei Hong, Lassen Emilie, Skytte Anne-Bine, Grońska-Pęski Marta, Shoag Jonathan E, Evrony Gilad D, Parsons Heather A, Mayer Erica L, Makrigiorgos G Mike, Golub Todd R, Adalsteinsson Viktor A
Abstract excerpt
Detecting mutations from single DNA molecules is crucial in many fields but challenging. Next-generation sequencing (NGS) affords tremendous throughput but cannot directly sequence double-stranded DNA molecules ('single duplexes') to discern the true mutations on both strands. Here we present Concatenating Original Duplex for Error Correction (CODEC), which confers single duplex resolution to NGS. CODEC affords...
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