Article

An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal stem cells

2023-10-10

Abstract excerpt

<h4>ABSTRACT</h4> <h4>Background and Aims</h4> The intestinal epithelium exhibits dynamic control of cell cycle phase lengths, yet no experimental platform exists for directly analyzing cell cycle phases in living human intestinal stem cells (ISCs). Here, we develop primary human ISC lines with two different reporter constructs to provide fluorescent readouts to analyze cell cycle phases in cycling ISCs. <h4>Met...

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Literature Corpus work: d2ad39d8-2de1-56fb-8a80-00a154d50cf0
DOI: 10.1101/2023.10.09.561410

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