Article

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

2024-12-22

Abstract excerpt

Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b5e32136-4e51-5445-b89c-c16ef6d5d08f
DOI: 10.1101/2024.12.19.629259

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studiesDOI 10.1101/2024.12.19.629259Read

Methods and sensors for functional genomic studies of cell-cycle transitions in single cells.Semantic proximity Read

Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes.Semantic proximity Read

Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell DyesSemantic proximity Read

An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal stem cellsSemantic proximity Read

Cumulative microscopy reveals cellular states in fibroblasts from patients with genetic disordersSemantic proximity Read

The Cell-Cycle State of Stem Cells Determines Cell Fate PropensitySemantic proximity Read

Characterising live cell behaviour: Traditional label-free and quantitative phase imaging approaches.Semantic proximity Read

MSCProfiler: An image processing workflow to investigate Mesenchymal Stem Cell heterogeneity using imaging flow cytometry dataSemantic proximity Read

Label-Free In-Line Characterization of Immune Cell Culture using Quantitative Phase ImagingSemantic proximity Read

Intensity Modulation of Trichromatic Split Fluorescent Proteins for Live Cell MappingSemantic proximity Read

FUCCI-based live imaging platform reveals cell cycle dynamics and identifies pro-proliferative compounds in human iPSC-derived cardiomyocytesSemantic proximity Read

High accuracy label-free classification of kinetic cell states from holographic cytometrySemantic proximity Read