Article
CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies.
Nature communications - 1 Aug 2026
Di Sante Moises, Pezzotti Melissa, Zimmermann Julius, Enrico Alessandro, Deschamps Joran, Balmas Elisa, Becca Silvia, Torchia Eloisa, Ballio Giulia, Solito Samantha, Sarchi Martina, Reali Alessandro, Bertero Alessandro, Jug Florian, Pasqualini Francesco S
Abstract excerpt
Cell-cycle progression is a major source of variability in live-cell phenotyping, yet imaging workflows still lack a general way to account for it alongside structural and functional readouts. Here we show CALIPERS (Cell-cycle-Aware Live-cell Imaging for Phenotyping Experiments and Regeneration Studies), an integrated framework that pairs a spectrally redesigned FUCCI reporter with continuous cell-cycle inference...
Read the complete abstract on PubMedTopics
- Humans
- Cell Cycle
- Regeneration
- Phenotype
- Induced Pluripotent Stem Cells