Article

LEMONmethyl-seq: Targeted long-read DNA methylation profiling reveals dynamics of CRISPR epigenome editing and endogenous DNA methylation patterns

2026-02-25

Abstract excerpt

<h4>BACKGROUND</h4> DNA methylation is the most prevalent epigenetic modification in human cells and undergoes dynamic changes during cell differentiation, disease progression, and aging. Here, we introduce L ocus- E nriched M apping O f N ucleotide methylation (LEMONmethyl-seq): an optimized, cost-effective pipeline for single-nucleotide detection of DNA methylation using locus-specific amplification and lo...

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Literature Corpus work: d2a06598-dbae-5265-b139-97656f275a0a
DOI: 10.64898/2026.02.24.707761

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