Article

Q-DOAS: A Proximity Quenching Assay for Real-Time Detection of Early Protein Aggregation Events

2026-06-17

Abstract excerpt

Accurate measurement of protein aggregation is essential for studying neurodegenerative diseases. The standard ThT assay reports on amyloid formation but is blind to early oligomers and is prone to interference. We describe Q-DOAS, a plate-reader assay that quantifies protein self-assembly in real time via proximity-quenching of a single, site-specifically conjugated dye (BODIPY-TMR). Using mutant Huntingtin-exon...

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Literature Corpus work: d0980d3a-aaf4-5721-b3c7-940ff79c4c41
DOI: 10.64898/2026.06.16.732424

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