Article

Cytotoxicity of Activator Expression in CRISPR-based Transcriptional Activation Systems

2024-09-23

Abstract excerpt

CRISPR-based transcriptional activation (CRISPRa) has extensive research and clinical potential. Here, we show that commonly used CRISPRa systems can exhibit pronounced cytotoxicity. We demonstrate the toxicity of published and new CRISPRa vectors expressing the activation domains (ADs) of the transcription factors p65 and HSF1, components of the synergistic activation mediator (SAM) CRISPRa system. Based on our f...

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Literature Corpus work: cff1dedf-9eab-5c51-9c62-cef9932ebbb3
DOI: 10.1101/2024.09.23.614524

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