Article

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

2023-11-10

Abstract excerpt

CRISPR-mediated gene activation (CRISPRa) encompasses a growing field of biotechnological approaches with exciting implications for gene therapy. However, there is a lack of experimental validation tools for selecting efficient sgRNAs for downstream applications. Here, we present a screening assay capable of identifying efficient single- and double sgRNAs through fluorescence quantification in vitro. In addition,...

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Literature Corpus work: 91803002-f814-5e89-b541-53ca309747c5
DOI: 10.1101/2023.11.10.566523

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