Article
CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.
Nature communications - 8 Jun 2018
Swings Toon, Marciano David C, Atri Benu, Bosserman Rachel E, Wang Chen, Leysen Marlies, Bonte Camille, Schalck Thomas, Furey Ian, Van den Bergh Bram, Verstraeten Natalie, Christie Peter J, Herman Christophe, Lichtarge Olivier, Michiels Jan
Abstract excerpt
CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad...
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