Article

A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes

2023-02-03

Abstract excerpt

Identifying virulence-critical genes from pathogens is often limited by functional redundancy. To rapidly interrogate the contributions of combinations of genes to a biological outcome, we have developed a mu ltiplex, randomized C RISPR interference s equencing (MuRCiS) approach. At its center is a new method for the randomized self-assembly of CRISPR arrays from synthetic oligonucleotide pairs. When paired wit...

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Literature Corpus work: c91559b3-a1da-5ffe-96b2-30ccf003b2b9
DOI: 10.1101/2023.02.03.527066

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