Article

Computational design of metalloproteases

2025-11-21

Abstract excerpt

Although significant progress has been made in creating de novo metalloenzymes that hydrolyze activated esters 1,2 , the energetically demanding cleavage of amide bonds has remained a major challenge for enzyme design: amide bonds are significantly more stable than ester bonds, the amine leaving groups in proteins are not activated, and peptide substrates are flexible making them difficult to bind precisely. Her...

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Literature Corpus work: c8562dc7-da5a-57c7-961d-4bf64b545e80
DOI: 10.1101/2025.11.20.689622

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