Article

Protease mimicry: dissecting the ester bond crosslinking mechanics in bacterial adhesin proteins

2025-03-27

Abstract excerpt

The ester bond crosslink discovered within bacterial adhesin proteins offers a captivating insight into the convergent evolution of enzyme-like machinery. Crystal structures reveal a putative catalytic triad comprising an acid-base-nucleophile combination and an oxyanion-like site that suggest a serine protease-like mechanism drives the crosslinking process. We now provide confirmation of the mechanism, revea...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c586e889-bc4b-5079-89ce-c6f751c203f3
DOI: 10.1101/2025.03.26.645365

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Protease mimicry: dissecting the ester bond crosslinking mechanics in bacterial adhesin proteinsDOI 10.1101/2025.03.26.645365Read

A highly unusual thioester bond in a pilus adhesin is required for efficient host cell interaction.Semantic proximity Read

Enzyme catalysis: a new definition accounting for noncovalent substrate- and product-like states.Semantic proximity Read

The unique functional role of the C-HS hydrogen bond in the substrate specificity and enzyme catalysis of type 1 methionine aminopeptidase.Semantic proximity Read

Structural and mechanistic exploration of acid resistance: kinetic stability facilitates evolution of extremophilic behavior.Semantic proximity Read

Conformational stability of the bacterial adhesin, FimH, with an inactivating mutation.Semantic proximity Read

Sequence diversity of NanA manifests in distinct enzyme kinetics and inhibitor susceptibility.Semantic proximity Read

Evolutionary paths that link orthogonal pairs of binding proteins.Semantic proximity Read

<i>Streptococcus pyogenes</i> nuclease A interferes with host complement functionsSemantic proximity Read

The reovirus sigma1 aspartic acid sandwich: a trimerization motif poised for conformational change.Semantic proximity Read

Surfactant-tolerance evolution of Bacillus clausii protease for enhancing activity and stability by reshaping the substrate access tunnel.Semantic proximity Read

X-ray structures of two active secreted <i>Bacteroides thetaiotaomicron</i> C11 proteases in complex with peptide-based inhibitorsSemantic proximity Read

Unique structural and ligand binding properties of the <i>Staphylococcus aureus</i> serine hydrolase FphESemantic proximity Read