Article

Optimizing a CRISPR-Cas13d gene circuit for tunable target RNA downregulation with minimal collateral RNA cutting

2024-05-11

Abstract excerpt

<h4>SUMMARY</h4> The invention of RNA-guided DNA cutting systems has revolutionized biotechnology. More recently, RNA-guided RNA cutting by Cas13d entered the scene as a highly promising alternative to RNA interference to engineer cellular transcriptomes for biotechnological and therapeutic purposes. Unfortunately, “collateral damage” by indiscriminate off-target cutting tampered enthusiasm for these systems. Yet...

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Literature Corpus work: 46a7693f-ac7b-5a53-96e0-fd70054c7a34
DOI: 10.1101/2024.05.11.593702

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