Article

Marker-free coselection for successive rounds of prime editing in human cells

2021-11-02

Abstract excerpt

<h4>ABSTRACT</h4> Prime editing enables the introduction of precise point mutations, small insertions, or short deletions without requiring donor DNA templates. However, efficiency remains a key challenge in a broad range of human cell types. In this work, we designed a robust coselection strategy through coediting of the ubiquitous and essential sodium/potassium pump (Na + /K + ATPase). We readily engineered h...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 17fdec64-0619-5db9-b2d3-3a05d01fa191
DOI: 10.1101/2021.11.02.464583

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Marker-free coselection for successive rounds of prime editing in human cellsDOI 10.1101/2021.11.02.464583Read

Marker-free co-selection for successive rounds of prime editing in human cellsSemantic proximity Read

A marker-free co-selection strategy for high efficiency human genome engineeringSemantic proximity Read

Optimized nickase- and nuclease-based prime editing in human and mouse cellsSemantic proximity Read

Efficient installation of heterozygous mutations in human pluripotent stem cells using prime editingSemantic proximity Read

Highly efficient generation of isogenic pluripotent stem cell models using prime editingSemantic proximity Read

Enrichment of allelic editing outcomes by Prime Editing in iPS cellsSemantic proximity Read

Programming large target genomic deletion and concurrent insertion via a prime editing-based method: PEDARSemantic proximity Read

Efficient and accurate prime editing strategy to correct genetic alterations in hiPSC using single EF-1alpha driven all-in-one plasmidsSemantic proximity Read

Mutation-specific reporter for optimization and enrichment of prime editingSemantic proximity Read

A refined Uni-vector prime editing system improves genome editing outcomes in mammalian cells.Semantic proximity Read

A homology independent sequence replacement strategy in human cells using a CRISPR nuclease.Semantic proximity Read

Mutation-specific reporter for optimization and enrichment of prime editing.Semantic proximity Read