Article

Interactomic affinity profiling by holdup assay: acetylation and distal residues impact the PDZome-binding specificity of PTEN phosphatase

2020-07-02

Abstract excerpt

Protein domains often recognize short linear protein motifs composed of a core conserved consensus sequence surrounded by less critical, modulatory positions. Here we used an accurate experimental approach combining high-throughput holdup chromatographic assay and fluorescence polarization to measure quantitative binding affinity profiles of the PDZ domain-binding motif (PBM) of PTEN phosphatase towards the 266 kn...

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Literature Corpus work: beb22dd7-e737-5ca3-a6ba-c1dae67a9cc1
DOI: 10.1101/2020.07.01.181487

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