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The Off-Target Effect of CRISPR-Cas12a System toward Insertions and Deletions between Target DNA and crRNA Sequences.

Analytical chemistry - 21 Jun 2022

Zhang Wei, Shi Rui, Dong Kejun, Hu Hao, Shu Wan, Mu Yaoqin, Yan Bei, Li Longjie, Xiao Xianjin, Wang Hongbo

Abstract excerpt

The CRISPR-Cas12a system is a new type of genome editing tool with high efficiency and targeting. However, other sequences in the genome may also be cleaved nonspecifically, resulting in unavoidable off-target effects. Therefore, it is necessary to learn more about the mechanism of CRISPR-Cas12a to recognize target sequences to avoid its off-target effects. Here, we show that insertion (DNA bubble) or deletion...

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PMID: 35670376
DOI: 10.1021/acs.analchem.1c05499

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Source receipt: pubmed, retrieved 7 Sept 2026.

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