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Article

Specificity profiling of CRISPR system reveals greatly enhanced off-target gene editing.

Scientific reports - 10 Feb 2020

Wang Yao, Wang Mingrui, Zheng Ting, Hou Yingzi, Zhang Pingjing, Tang Tao, Wei Jing, Du Quan

Abstract excerpt

To explore the editing specificity of CRISPR/Cpf1 system, effects of target mutation were systematically examined using a reporter activation assay, with a set of single-nucleotide mutated target site. Consistent with our previous study performed with CRISPR/Cas9, a "core" sequence region that is highly sensitive to target mutation was characterized. The region is of 4-nucleotide long, located from +4 to +7...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 32042045
DOI: 10.1038/s41598-020-58627-x
PMCID: PMC7010781

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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