Article

Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system

2016-02-19

Abstract excerpt

Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms. These new platforms, such as 454 and Illumina's MiSeq, have allowed researchers to obtain millions of high quality, but short sequences. The result of the added sequencing depth has been significant improvements in experimen...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bd49af11-65c8-5fb4-99ef-ffab888abc22
DOI: 10.7287/peerj.preprints.778v2

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing systemDOI 10.7287/peerj.preprints.778v2Read

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolutionSemantic proximity Read

Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification.Semantic proximity Read

The Characterization of Novel Tissue Microbiota Using an Optimized 16S Metagenomic Sequencing PipelineSemantic proximity Read

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in microbiome samplesSemantic proximity Read

Analysis of the cystic fibrosis lung microbiota via serial Illumina sequencing of bacterial 16S rRNA hypervariable regions.Semantic proximity Read

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samplesSemantic proximity Read

The impact of DNA polymerase and number of rounds of amplification in PCR on 16S rRNA gene sequence dataSemantic proximity Read

Improving bacterial metagenomic research through long read sequencingSemantic proximity Read

Accurate Determination of Bacterial Abundances in Human Metagenomes Using Full-length 16S Sequencing ReadsSemantic proximity Read

Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples.Semantic proximity Read

Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation.Semantic proximity Read

Accuracy and quality of massively parallel DNA pyrosequencing.Semantic proximity Read