Article
Analysis of the cystic fibrosis lung microbiota via serial Illumina sequencing of bacterial 16S rRNA hypervariable regions.
PloS one - 1 Jan 2012
Maughan Heather, Wang Pauline W, Diaz Caballero Julio, Fung Pauline, Gong Yunchen, Donaldson Sylva L, Yuan Lijie, Keshavjee Shaf, Zhang Yu, Yau Yvonne C W, Waters Valerie J, Tullis D Elizabeth, Hwang David M, Guttman David S
Abstract excerpt
The characterization of bacterial communities using DNA sequencing has revolutionized our ability to study microbes in nature and discover the ways in which microbial communities affect ecosystem functioning and human health. Here we describe Serial Illumina Sequencing (SI-Seq): a method for deep sequencing of the bacterial 16S rRNA gene using next-generation sequencing technology. SI-Seq serially sequences...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.