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Article

Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples.

PloS one - 1 Jan 2016

Barb Jennifer J, Oler Andrew J, Kim Hyung-Suk, Chalmers Natalia, Wallen Gwenyth R, Cashion Ann, Munson Peter J, Ames Nancy J

Abstract excerpt

OBJECTIVES: There is much speculation on which hypervariable region provides the highest bacterial specificity in 16S rRNA sequencing. The optimum solution to prevent bias and to obtain a comprehensive view of complex bacterial communities would be to sequence the entire 16S rRNA gene; however, this is not possible with second generation standard library design and short-read next-generation sequencing...

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Identifiers

PMID: 26829716
DOI: 10.1371/journal.pone.0148047
PMCID: PMC4734828

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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