Article
Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples.
PloS one - 1 Jan 2016
Barb Jennifer J, Oler Andrew J, Kim Hyung-Suk, Chalmers Natalia, Wallen Gwenyth R, Cashion Ann, Munson Peter J, Ames Nancy J
Abstract excerpt
OBJECTIVES: There is much speculation on which hypervariable region provides the highest bacterial specificity in 16S rRNA sequencing. The optimum solution to prevent bias and to obtain a comprehensive view of complex bacterial communities would be to sequence the entire 16S rRNA gene; however, t...
Topics
- Bacteria
- Computational Biology
- DNA Barcoding, Taxonomic
- Gene Order
- Genetic Loci
- Genetic Variation
- High-Throughput Nucleotide Sequencing
- Metagenomics
- Operon
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA