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Article

Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification.

BMC microbiology - 14 Nov 2016

Wagner Josef, Coupland Paul, Browne Hilary P, Lawley Trevor D, Francis Suzanna C, Parkhill Julian

Abstract excerpt

BACKGROUND: Currently, bacterial 16S rRNA gene analyses are based on sequencing of individual variable regions of the 16S rRNA gene (Kozich, et al Appl Environ Microbiol 79:5112-5120, 2013).This short read approach can introduce biases. Thus, full-length bacterial 16S rRNA gene sequencing is needed to reduced biases. A new alternative for full-length bacterial 16S rRNA gene sequencing is offered by PacBio single...

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Identifiers

PMID: 27842515
DOI: 10.1186/s12866-016-0891-4
PMCID: PMC5109829

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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