Article

The impact of DNA polymerase and number of rounds of amplification in PCR on 16S rRNA gene sequence data

2019-03-04

Abstract excerpt

PCR amplification of 16S rRNA genes is a critical, yet under appreciated step in the generation of sequence data to describe the taxonomic composition of microbial communities. Numerous factors in the design of PCR can impact the sequencing error rate, the abundance of chimeric sequences, and the degree to which the fragments in the product represent their abundance in the original sample (i.e. bias). We compared...

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Literature Corpus work: c45f0c43-c82a-519b-b2c5-db45b448fede
DOI: 10.1101/565598

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