Article

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

2018-08-15

Abstract excerpt

Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widely used to profile microbial communities. New long-read sequencing technologies can sequence the entire 16S rRNA gene, but higher error rates have limited their attractiveness when accuracy is important. Here we present a high-throughput amplicon sequencing methodology based on PacBio circular consensu...

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Literature Corpus work: 314643c7-3943-5688-86fd-4cf50f292767
DOI: 10.1101/392332

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