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Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

25 Mar 2024

Abstract excerpt

BACKGROUND: Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human microbiota. Unfortunately, short reads are unable to differentiate between highly similar species. Considering that species from the same genus can be associated with health or disease it is important to identify them at the lowest possible taxonomic rank....

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PMID: 38528457
DOI: 10.1186/s12864-024-10213-5

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