Article
Accuracy and quality of massively parallel DNA pyrosequencing.
Genome biology - 1 Jan 2007
Huse Susan M, Huber Julie A, Morrison Hilary G, Sogin Mitchell L, Welch David Mark
Abstract excerpt
BACKGROUND: Massively parallel pyrosequencing systems have increased the efficiency of DNA sequencing, although the published per-base accuracy of a Roche GS20 is only 96%. In genome projects, highly redundant consensus assemblies can compensate for sequencing errors. In contrast, studies of microbial diversity that catalogue differences between PCR amplicons of ribosomal RNA genes (rDNA) or other conserved gene...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.