Article

Structure of human Mediator-RNA polymerase II transcription pre-initiation complex

2021-03-12

Abstract excerpt

Mediator is a conserved coactivator that enables regulated transcription initiation from eukaryotic protein-coding genes 1-3 . Mediator is recruited by transcriptional activators and binds the pre-initiation complex (PIC) to stimulate RNA polymerase II (Pol II) phosphorylation and promoter escape 1-6 . Here we prepare a 20-subunit recombinant human Mediator, reconstitute a 50-subunit Mediator-PIC complex, and re...

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Literature Corpus work: bb41e029-6fb1-570a-8b04-793f83eacd8d
DOI: 10.1101/2021.03.11.435010

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