Article

Translation of numerous upstream open reading frames rather than their products is essential for proliferation of human cells of distinct origin

2025-07-07

Abstract excerpt

Upstream open reading frames (uORFs) are a widespread class of translated regions (translons) occurring in 5′ leaders of mRNAs and serving critical roles in post-transcriptional regulation. However, their specific biological activities in human cells remains to be fully elucidated. Here, we conducted a genome-wide CRISPR-Cas9 loss-of-function screen of 978 uORFs identified with ribosome profiling, across human cel...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b89c9710-5242-57e0-9ce1-738d74be4408
DOI: 10.1101/2025.07.07.663426

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Translation of numerous upstream open reading frames rather than their products is essential for proliferation of human cells of distinct originDOI 10.1101/2025.07.07.663426Read

Gene expression regulation by upstream open reading frames and human disease.Semantic proximity Read

Sequence-intrinsic barriers define a class of translation-restricted uORFs in the human genomeSemantic proximity Read

uORFdb--a comprehensive literature database on eukaryotic uORF biology.Semantic proximity Read

Disrupting upstream translation in mRNAs is associated with human disease.Semantic proximity Read

Some like it translated: small ORFs in the 5′UTRSemantic proximity Read

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans.Semantic proximity Read

Disrupting upstream translation in mRNAs leads to loss-of-function associated with human diseaseSemantic proximity Read

A comprehensive catalog of predicted functional upstream open reading frames in humans.Semantic proximity Read

An expanded reference catalog of translated open reading frames for biomedical researchSemantic proximity Read

Translational effects and coding potential of an upstream open reading frame associated with DOPA Responsive Dystonia.Semantic proximity Read

Analysis of human upstream open reading frames and impact on gene expression.Semantic proximity Read

Autonomous functionality of an upstream open reading frame in polycistronic mammalian mRNAsSemantic proximity Read