Article

Linked-Pair Long-Read Sequencing Strategy for Targeted Resequencing and Enrichment

2023-10-31

Abstract excerpt

<h4>ABSTRACT</h4> In this report, we present linked-pair sequencing, a novel strategy to construct a long-read sequencing library such that adjacent fragments are linked with end-terminal duplications. We use the CRISPR-Cas9 nickase enzyme and a pool of multiple sgRNAs to perform non-random fragmentation of targeted long DNA molecules (>300kb) into smaller library-sized fragments (about 20 kbp) in a manner so as...

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Literature Corpus work: b7de6d09-8d1e-59f2-bc1b-5376e755c207
DOI: 10.1101/2023.10.26.564243

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