Article
CRISPR-Cas9 Targeted Enrichment and Next-Generation Sequencing for Mutation Detection.
The Journal of molecular diagnostics : JMD - 1 May 2023
Malekshoar Mehrdad, Azimi Sajad Ataei, Kaki Arastoo, Mousazadeh Leila, Motaei Jamshid, Vatankhah Majid
Abstract excerpt
Despite the rapid application of next-generation sequencing (NGS) technologies, target sequencing in regions of the genome is often required to diagnose many genetic diseases. Target enrichment can be an effective factor in reducing the cost of sequencing and the duration of sequencing. Recently, several clustered system regularly interspaced short palindromic repeats (CRISPR)-based methods (amplification-free...
Topics
- Humans
- CRISPR-Cas Systems
- Mutation
- DNA
- Genomics
- High-Throughput Nucleotide Sequencing