Article

Computationally Engineered CRISPR-SpyCas9 High-Fidelity Variants with Improved Specificity and Reduced Non-specific DNA Damage

2023-04-11

Abstract excerpt

<h4>ABSTRACT</h4> The CRISPR-Cas system holds great promise in the treatment of diseases caused by genetic variations. As wildtype SpyCas9 is known to generate many off-target effects, its use in the clinic remains controversial due to safety concerns. Several high-fidelity Cas9 variants with greater specificity have been developed using rational design and directed evolution. However, the enhancement of specific...

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Literature Corpus work: b79be0f9-ec91-57d7-a9ae-f24fec473214
DOI: 10.1101/2023.04.11.536265

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