Article

Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9

2023-12-15

Abstract excerpt

<h4>SUMMARY</h4> Thermostable CRISPR-Cas9 enzymes could improve genome editing efficiency and delivery due to extended protein lifetimes. However, initial experimentation demonstrated Geobacillus stearothermophilus Cas9 (GeoCas9) to be virtually inactive when used in cultured human cells. Laboratory-evolved variants of GeoCas9 overcome this natural limitation by acquiring mutations in the wedge (WED) domain that...

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Literature Corpus work: 9ff2850a-86ac-537e-8a28-d34b87bd40b1
DOI: 10.1101/2023.12.14.571777

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