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Article

Protein-Mutation-Induced Conformational Changes of the DNA and Nuclease Domain in CRISPR/Cas9 Systems by Molecular Dynamics Simulations.

The journal of physical chemistry. B - 19 Mar 2020

Ray Angana, Di Felice Rosa

Abstract excerpt

Class 2 CRISPR (clustered regularly interspaced short palindromic repeats) systems offer a unique protocol for genome editing in eukaryotic cells. The nuclease activity of Cas9 has been harnessed to perform precise genome editing by creating double-strand breaks. However, the nuclease activity of Cas9 can be triggered when there is imperfect complementarity between the RNA guide sequence and an off-target genomic...

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PMID: 32079396
DOI: 10.1021/acs.jpcb.9b07722

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