Using CRISPR/Cas9 Technology for Manipulating Cell Death Regulators.

Abstract excerpt

Clustered, regularly interspaced, short palindromic repeats (CRISPR)/Cas9 technology has been demonstrated to be a useful tool for generating targeted mutations in cell lines and mice. However, the use of CRISPR/Cas9 in a constitutively expressed manner can often result in low targeting efficiencies and lethality due to mutations in essential genes. Here, we describe the use of an inducible lentiviral vector...

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