Article

Allele-resolved monosome and polysome sequencing identifies functional cis-acting variants affecting mRNA translation efficiency

2026-05-11

Abstract excerpt

To prioritize germline genetic variants affecting mRNA fate at the post-transcriptional and translational levels, we leveraged sucrose-gradient-based isolation of 80S monosomes and polysomes, followed by mRNA retrieval and paired-end sequencing. Total cytoplasmic RNA was also sequenced for comparison. Experiments were performed in the non-transformed cell line RPE-1, cultured under basal conditions or upon p53 act...

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Literature Corpus work: b6876d70-2d80-5384-be56-154d186f7ca8
DOI: 10.64898/2026.05.07.723424

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