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Article

Polysome-profiling in small tissue samples.

Nucleic acids research - 9 Jan 2018

Liang Shuo, Bellato Hermano Martins, Lorent Julie, Lupinacci Fernanda C S, Oertlin Christian, van Hoef Vincent, Andrade Victor P, Roffé Martín, Masvidal Laia, Hajj Glaucia N M, Larsson Ola

Abstract excerpt

Polysome-profiling is commonly used to study translatomes and applies laborious extraction of efficiently translated mRNA (associated with >3 ribosomes) from a large volume across many fractions. This property makes polysome-profiling inconvenient for larger experimental designs or samples with low RNA amounts. To address this, we optimized a non-linear sucrose gradient which reproducibly enriches for efficiently...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 29069469
DOI: 10.1093/nar/gkx940
PMCID: PMC5758873

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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