Article

Single-cell Genome-and-Transcriptome sequencing without upfront whole-genome amplification reveals cell state plasticity of melanoma subclones

2023-01-13

Abstract excerpt

<h4>ABSTRACT</h4> Single-cell multi-omics methods are enabling the study of cell state diversity, which is largely determined by the interplay of the genome, epigenome, and transcriptome. Here, we describe Gtag&T-seq, a genome-and-transcriptome sequencing (G&T-seq) protocol of the same single cells that omits whole-genome amplification (WGA) by using direct genomic tagmentation (Gtag). Gtag drastically decreases...

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Literature Corpus work: b4ec5e34-4ef2-5c25-8ecb-10c0851d2c4a
DOI: 10.1101/2023.01.13.521174

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