Article
Using CRISPR to understand and manipulate gene regulation.
Development (Cambridge, England) - 1 May 2021
Akinci Ersin, Hamilton Marisa C, Khowpinitchai Benyapa, Sherwood Richard I
Abstract excerpt
Understanding how genes are expressed in the correct cell types and at the correct level is a key goal of developmental biology research. Gene regulation has traditionally been approached largely through observational methods, whereas perturbational approaches have lacked precision. CRISPR-Cas9 has begun to transform the study of gene regulation, allowing for precise manipulation of genomic sequences, epigenetic...
Topics
- Animals
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- DNA
- Epigenomics
- Gene Editing
- Gene Expression Regulation
- Gene Regulatory Networks
- Humans
- Mutation