Article

Revisiting the impact of synthetic ORF sequences on engineered LINE-1 retrotransposition

2022-08-29

Abstract excerpt

The retrotransposon Long Interspersed Element-1 (L1) contains adenosine rich open reading frames (ORFs), a characteristic that limits its expression in mammalian cells. A synthetic mouse L1 (smL1) with ORF adenosine content decreased from 40% to 26% showed increased mRNA expression and retrotransposed far more efficiently than the native parental element, L1spa (1). Here, we observe two nonsynonymous substitutions...

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Literature Corpus work: abfc3829-f392-529f-bc18-cdc5a2f13e9c
DOI: 10.1101/2022.08.29.505632

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