Article

Droplet single-cell CRISPR screens identify regulators of T cell–mediated target-cell killing

2026-06-29

Abstract excerpt

Cytotoxic CD8⁺ T cells kill target cells through brief cell–cell encounters, but pooled genetic screens cannot readily link perturbations in individual T cells to the fate of the target cells they engage. We developed droplet single-cell CRISPR screening to pair individual primary human CD8⁺ T cells with cancer cells, measure rapid target-cell death, and recover sgRNAs from phenotype-defined droplets. Applied acro...

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Literature Corpus work: a946fd32-ba8d-5778-ab58-9dbe86762244
DOI: 10.64898/2026.06.23.734054

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