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Chemically synthesized guide RNAs can direct CRISPR-CasRx cleavage of circRNAs with high efficiency and specificity

2022-08-30

Abstract excerpt

Circular RNAs (circRNAs) are characterized by a covalently closed circular structure, formed from pre-mRNAs through an alternative splicing mechanism named back-splicing. CircRNAs have been shown to play a regulatory role in the development of eukaryotic organisms and to be implicated in human diseases. However, the extensive sequence-overlap between circRNAs and their linear RNA counterparts makes it technically...

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Literature Corpus work
ecf7ba90-57f7-5a0f-83a5-3d00312ad5f9
DOI
10.1101/2022.08.30.505797
Open publication

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Chemically synthesized guide RNAs can direct CRISPR-CasRx cleavage of circRNAs with high efficiency and specificityDOI 10.1101/2022.08.30.505797
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