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Characteristics, Recombination Methods, and Applications Progresses of Split-Cas9 System

2 Jun 2023

Abstract excerpt

CRISPR technology has been used to revolutionize various facets of life sciences because of its potent gene editing capabilities. In particular, CRISPR technology is anticipated to be used to cure congenital disorders, and malignant cancers brought on by gene mutation. In this article, we introduce a Split-Cas9 system, in which Cas9 protein is split into two or more parts and recombined in cells to function...

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PMID: 37265143
DOI: 10.1089/hum.2022.223

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