Article
The novel insight into the outcomes of CRISPR/Cas9 editing intra- and inter-species.
International journal of biological macromolecules - 15 Nov 2020
Chang Jiasong, Chen Xiaoxu, Zhang Tong, Wang Ruolin, Wang Aoming, Lan Xinhui, Zhou Yuyu, Ma Sanyuan, Xia Qingyou
Abstract excerpt
The CRISPR/Cas (clustered regularly interspaced short palindromic repeat technology/CRISPR-associated protein) is a widely used and powerful research tool in biosciences and a promising therapeutic agent for treating genetic diseases. Mutations induced by Cas9 are generally considered stochastic and unpredictable, thus hindering its applications where precise genetic alternations are required. Here, through deep...
Topics
- Animals
- CRISPR-Cas Systems
- Cell Line
- Gene Editing
- Genetic Vectors
- High-Throughput Nucleotide Sequencing
- Humans
- INDEL Mutation
- Mice
- Mutation
- RNA, Guide, CRISPR-Cas Systems